5808 N. Attleburg Dr. McCordsville, IN 46055-9225
A Research Scientist with 18 years of experience at Indiana University School of Medicine. Published 13 articles in scientific journals, presented my results at 3 national meetings and was awarded two research grants . A proven ability to manage and maintain an HPLC laboratory with a tight NIH budget. Gained experience in biochemical and molecular biological methods
Research Associate, 12/2000-7/2008 Indiana University School of Medicine, Indianapolis, IN
Managed and maintained an HPLC laboratory. Routinely analyzed for amino acids (including DL-AA) from tissue and plasma; adenine nucleotides from tissue and tissue culture. Also, ran analysis for catecholamines, keto acids, and drug concentration in brain tissue.
Assistant Scientist, 6/1993-11/2008 Indiana University School of Medicine, Indianapolis, IN
Researched the mechanisms of pulmonary diseases using biochemical and molecular biological methods. Mentored pulmonary fellows in their laboratory research.
Techniques: isolated proteins fibronectin and vitronectin, DNA synthesis, PCR, tissue culture, western blot.
Laboratory Technician, 9/1979-8/1980 Baylor College of Medicine, Houston, TX
Tissue culture. Developed ELISA assays for influenza and adenovirus. Isolated antibodies and influenza virus.
Pike Township Little League Summer 2000, 01, 02 Indianapolis IN
Cooked hotdogs, hamburgers, and bratwurst during the games.
Postdoctoral Fellow, 5/1990-5/1993 Indiana University School of Medicine, Indianapolis, IN
Research mechanisms of pulmonary disease using biochemical and molecular biological methods.
PhD, Biochemistry, 1/1983-5/1990 Purdue University, West Lafayette, IN
Thesis: Site directed mutagenesis and characterization of the lactose repressor. Techniques: standard recombinant DNA protocols (including site directed mutagenesis, RNA and DNA isolation, PCR, and M-13 sequencing), protein isolation, viral isolation, fermentation, tissue culture, protein-DNA interactions and electrophoresis (DNA, RNA and protein).
MS, Chemistry, 9/1980-9/1982 Bowling Green State University, Bowling Green, OH
Thesis: Isolation and characterization of a plant alkaloid. Techniques: Thin Layer Chromatography, High Pressure Liquid Chromatography, Nuclear Magnetic Resonance, Infrared Spectroscopy, and Mass Spectrometry.
BS, Biochemistry, 9/1975-5/1979 University of Massachusetts, Amherst, MA
Windows, Word ,Excel, Empower
Shen W, Boyle DW, Wisniowski P, Bade A, Liechty EA. Insulin and IGF-I stimulate the formation of the eukaryotic initiation factor 4F complex and protein synthesis in C2C12 myotubes independent of availability of external amino acids. J. of Endocrinology 185: 275-289, 2005.
Shen W, Wisniowski P, Ahmed L, Boyle DW, Denne SC, Liechty EA. Anabolic effects of insulin and IGF-1 in the ovine fetus are reduced by prolonged maternal fasting. Am. J. of Physiol
288: E907-E913, 2005.
Shen W, Wisniowski P, Ahmed L, Boyle DW, Denne SC, Liechty EA. Protein anabolic effects of insulin and IGF-1 ovine fetus Am. J. of Physiol. 284:E787-E756, 2003.
Pasula R, Wisniowski P, and Martin WJ II. Fibronectin facilitates Mycobacterium tuberculosis attachment to murine alveolar macrophages. Infection and Immunity 70(3):1287-92, 2002.
Wisniowski P, Spech RW, Wu M, Doyle N, Pasula R and Martin WJ II. Vitronectin protects alveolar macrophages from silica toxicity. Am. J. Respir. Crit. Care Med. 162:733-739, 2000.
Spech RW, Wisniowski P, Kachel DL, Wright JR, and Martin WJ II. Surfactant protein A prevents silica-mediated toxicity to rat alveolar macrophages. Am J. of Physiol. 278:L713-L718, 2000.
Perry DG, Wisniowski P, Downing J, and Martin WJ II. Nonimmune phagocytosis of liposomes by rat alveolar macrophages is enhanced by vitronectin and is vitronectin-mediated. Am. J. Respir. Cell Mol. Biol. 17:462-470, 1997.
Weaver T, Hall CL, Ward R, Kachel DL, Williams MD, Perry DG, Wisniowski P, and Martin WJ II. Assessment of in vivo attachment/phagocytosis by alveolar macrophages. J. Immunol Meth. 193:149-156, 1996.
Wisniowski P, Pasula R, and Martin WJ II. Interaction of vitronectin with Pneumocystis carinii: Evidence for binding via heparin binding domain. J. Lab. Clin. Med. 125:38-45, 1995.
Wisniowski P, Pasula R, and Martin WJ II. Isolation of Pneumocystis carinii gp120 by fibronectin affinity: Evidence for manganese dependence. Am. J. Respir. Cell Mol. Biol. 11(3):262-269, 1994.
Pottratz ST, Weir A, Wisniowski PE. Pneumocystis carinii attachment increases expression of fibronectin-binding integrins on cultured lung cells. Infection and Immunity.
Basile F, Hughes KD, Wisniowski PE, Gorenstein DG, Lytle FE, McCay-Buis TS, Huber DM, and Hemming BC. Fast and sensitive laser-based enzymatic detection of the lactose operon in microorganisms. Anal. Biochem. 221:55-60, 1993.
Wisniowski P, Karslake C, Piotto MF, Spangler B, Moulin A-C, Nikonowicz E, Kaluarchchi K, and Gorenstein DG. Importance of phosphate ester backbone flexibility in protein recognition. Structure & Function (Sarma, R.H., and Sarma, M.H., eds.) pp 17-54, Adenine Press, Schenectady NY, 1992.
Karslake C, P. Wisniowski, B-D Spangler, A.-C. Moulin, P. L. Wang and D. G. Gorenstein. Construction, DNA binding, two-dimensional nuclear magnetic resonance and structure of a mutant Lac repressor headpiece. J. Am.. Chem. Soc., 113, 4003-4005, 1991.